![]() Currently, 19.5% of the irrigated farmland areas is contaminated by salinity ( ). Soil salinization is one of the major abiotic stresses that limit plant growth and development, leading to excessive reduction in global agricultural production, especially in arid and semi-arid areas. These data provide the first set of full-length isoforms in okra and more comprehensive insights into the molecular mechanism of melatonin responses to salt stress. Our preliminary results suggested that melatonin pretreatment enhanced salt tolerance of okra plants for the first time. Many transcription factors including MYB, WRKY, NAC etc., were also identified as DEGs. Nitrogen metabolism, sulfur metabolism, and alanine, aspartate and glutamate metabolism were significantly enriched in all three treatments. These genes were enriched in more than 10 GO terms and 34 KEGG pathways. Illumina RNA-seq analysis showed that: Comparing with control, 1776, 10 differential expression genes (DEGs) were identified from the three treatments (NaCl, MT50 and MT + NaCl, respectively). A total of 121,360 unigenes was obtained, and the size of transcript lengths ranged from 500 to 6000 bp. Moreover, a combination of third- (PacBio) and second-generation (Illumina) sequencing technologies was applied to sequence full-length transcriptomes of okra. - in melatonin-pretreated plants, which may be attributed to the higher levels of enzyme activities including POD and GR.Additionally, melatonin pretreatment remarkably decreased the levels of lipid peroxidation and H 2O 2 content and scavenged O 2 Results showed that exogenous application of melatonin (50 μM) significantly enhanced plant tolerance to salt stress, as demonstrated by the plant resistant phenotype, as well as by the higher levels of the net photosynthetic rate, chlorophyll fluorescence and chlorophyll content in comparison with nontreated salt-stressed plants. In this study, we explored whether exogenous melatonin pretreatment could alleviate salt stress (300 mM NaCl) of okra plants. However, little is known about its regulatory mechanisms in response to salt stress in okra. Recent reports have revealed the protective role of melatonin in alleviating salt stress. Okra ( Abelmoschus esculentus L.) is a food crop with extremely high values of nutrition and healthcare. Melatonin, a multifunctional signal molecule, has been reported to play crucial roles in growth and development and stress responses in various plant species.
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